Peanut allergy: Determining peanut allergens with ELISA
An Innovative Testing research contribution was accepted for publication  this week in The Journal of Agricultural and Food Chemistry. First author Jayasena, together with others describes the comparison of six commercially available methods to determine the content of various peanut allergens in peanut butter. The results in the article provide indications for which methods is best for determining the most potent allergens and which of the methods can be used best for other peanut allergens. Below you will find the technical abstract and full reference of the article.
Six commercial peanut ELISA kits were assessed for their ability to recover peanut from the standard reference material (SRM) 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3 and Ara h 6. The percentage recovery of peanut from peanut butter differed across different kits as well as at different sample concentrations. The highest recovery was observed with the Romer and R-Biopharm kits while four other kits were found to underestimate the protein content of the reference peanut butter samples. Five of the kits were most sensitive in detecting Ara h 3 followed by Ara h 1, while hardly recognizing Ara h 2 and Ara h 6. The other kit showed the highest sensitivity to Ara h and Ara h 6, while Ara h 1 and Ara h 3 were poorly recognized. Although Ara h 2 and Ara h 6 are known to be heat stable and more potent allergens, antisera specific to any of these four peanut proteins/allergens may serve as good markers for the detection of peanut residues.
1) Jayasena, Mieke Smits, Daniëlle Fiechter, Aard de Jong, Julie Nordlee, Joe Baumert, Steve L. Taylor, Raymond H. Pieters, and Stef J. Koppelmanet al., Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens. Journal of Agricultural and Food Chemistry, 2015.